Research

Publications by Amy K. Cain

Check out our latest publications on Google Scholar or Pubmed.

RSS Cain AK

  • Shape-based separation of drug-treated <em>Escherichia coli</em> using viscoelastic microfluidics June 1, 2022
    Here, we achieve shape-based separation of drug-treated Escherichia coli (E. coli) by viscoelastic microfluidics. Since shape is critical for modulating biological functions of E. coli, the ability to prepare homogeneous E. coli populations adopting uniform shape or sort bacterial sub-population based on their shape has significant implications for a broad range of biological, biomedical and […]
    Tianlong Zhang
  • Genomic and phenotypic analyses of diverse non-clinical <em>Acinetobacter baumannii</em> strains reveals strain-specific virulence and resistance capacity February 15, 2022
    Acinetobacter baumannii is a critically important pathogen known for its widespread antibiotic resistance and ability to persist in hospital-associated environments. Whilst the majority of A. baumannii infections are hospital-acquired, infections from outside the hospital have been reported with high mortality. Despite this, little is known about the natural environmental reservoir(s) of A. baumannii and the […]
    Mohammad Hamidian
  • Length-based separation of <em>Bacillus subtilis</em> bacterial populations by viscoelastic microfluidics February 7, 2022
    In this study, we demonstrated the label-free continuous separation and enrichment of Bacillus subtilis populations based on length using viscoelastic microfluidics. B. subtilis, a gram-positive, rod-shaped bacterium, has been widely used as a model organism and an industrial workhorse. B. subtilis can be arranged in different morphological forms, such as single rods, chains, and clumps, […]
    Ping Liu
  • Genomic investigation of a suspected <em>Klebsiella pneumoniae</em> outbreak in a neonatal care unit in sub-Saharan Africa November 18, 2021
    A special-care neonatal unit from a large public hospital in Malawi was noted as having more frequent, difficult-to-treat infections, and a suspected outbreak of multi-drug-resistant Klebsiella pneumoniae was investigated using genomic characterisation. All K. pneumoniae bloodstream infections (BSIs) from patients in the neonatal ward (n=62), and a subset of K. pneumoniae BSI isolates (n=38) from […]
    Jennifer Cornick
  • Editorial: Secondary Effects of Antibiotic Exposure September 10, 2021
    No abstract
    Amy K Cain
  • The Molecular Basis of Acinetobacter baumannii Cadmium Toxicity and Resistance September 8, 2021
    Acinetobacter species are ubiquitous Gram-negative bacteria that can be found in water, in soil, and as commensals of the human skin. The successful inhabitation of Acinetobacter species in diverse environments is primarily attributable to the expression of an arsenal of stress resistance determinants, which includes an extensive repertoire of metal ion efflux systems. Metal ion […]
    Saleh F Alquethamy
  • Identification of Tse8 as a Type VI secretion system toxin from Pseudomonas aeruginosa that targets the bacterial transamidosome to inhibit protein synthesis in prey cells August 20, 2021
    The Type VI secretion system (T6SS) is a bacterial nanomachine that delivers toxic effectors to kill competitors or subvert some of their key functions. Here, we use transposon directed insertion-site sequencing to identify T6SS toxins associated with the H1-T6SS, one of the three T6SS machines found in Pseudomonas aeruginosa. This approach identified several putative toxin-immunity […]
    Laura M Nolan
  • Splicing factor proline and glutamine rich intron retention, reduced expression and aggregate formation are pathological features of amyotrophic lateral sclerosis July 21, 2021
    CONCLUSIONS: Our findings confirm dysregulation of SFPQ as a pathological feature of the central nervous system of ALS patients and indicate that investigation of the functional consequences of this pathology will provide insight into ALS biology.
    Alison L Hogan
  • <em>Acinetobacter baumannii</em> Fatty Acid Desaturases Facilitate Survival in Distinct Environments June 8, 2021
    Maintaining optimal fluidity is essential to ensure adequate membrane structure and function under different environmental conditions. We apply integrated molecular approaches to characterize two desaturases (DesA and DesB) and define their specific roles in unsaturated fatty acid (UFA) production in Acinetobacter baumannii. Using a murine model, we reveal DesA to play a minor role in […]
    Felise G Adams
  • Platinum Cyclooctadiene Complexes with Activity against Gram-positive Bacteria May 21, 2021
    Antimicrobial resistance is a looming health crisis, and it is becoming increasingly clear that organic chemistry alone is not sufficient to continue to provide the world with novel and effective antibiotics. Recently there has been an increased number of reports describing promising antimicrobial properties of metal-containing compounds. Platinum complexes are well known in the field […]
    Angelo Frei
  • Microbiology's next top model: Galleria in the molecular age January 21, 2021
    Galleria mellonella has risen to fame as an invertebrate model organism given its ethical advantages, low maintenance costs, rapid reproduction time, short life cycle, high number of progeny, tolerance for human body temperatures, innate immune system and similarities to mammalian host models. It is increasingly being utilised to evaluate in vivo toxicity and efficacy of […]
    Hue Dinh
  • Elucidating Essential Genes in Plant-Associated Pseudomonas protegens Pf-5 Using Transposon Insertion Sequencing December 1, 2020
    Gene essentiality studies have been performed on numerous bacterial pathogens, but essential gene sets have been determined for only a few plant-associated bacteria. Pseudomonas protegens Pf-5 is a plant-commensal, biocontrol bacterium that can control disease-causing pathogens on a wide range of crops. Work on Pf-5 has mostly focused on secondary metabolism and biocontrol genes, but […]
    Belinda K Fabian
  • Nontoxic Cobalt(III) Schiff Base Complexes with Broad-Spectrum Antifungal Activity November 24, 2020
    Resistance to currently available antifungal drugs has quietly been on the rise but overshadowed by the alarming spread of antibacterial resistance. There is a striking lack of attention to the threat of drug-resistant fungal infections, with only a handful of new drugs currently in development. Given that metal complexes have proven to be useful new […]
    Angelo Frei
  • The Transcriptomic Signature of Tigecycline in <em>Acinetobacter baumannii</em> November 16, 2020
    Tigecycline, a protein translation inhibitor, is a treatment of last resort for infections caused by the opportunistic multidrug resistance human pathogen Acinetobacter baumannii. However, strains resistant to tigecycline were reported not long after its clinical introduction. Translation inhibitor antibiotics perturb ribosome function and induce the reduction of (p)ppGpp, an alarmone involved in the stringent response […]
    Liping Li
  • How antibiotics work together: molecular mechanisms behind combination therapy July 4, 2020
    Antibiotics used in combination are an effective strategy for combatting numerous infectious diseases in clinical and veterinary settings, particularly as a last-line therapy for difficult-to-treat cases. Combination therapy can either increase or slow the rate of killing, broaden the antibiotic spectrum, reduce dosage and unwanted side-effects, and even control the emergence of resistance. The administration […]
    Geraldine J Sullivan
  • An amphipathic peptide with antibiotic activity against multidrug-resistant Gram-negative bacteria June 25, 2020
    Peptide antibiotics are an abundant and synthetically tractable source of molecular diversity, but they are often cationic and can be cytotoxic, nephrotoxic and/or ototoxic, which has limited their clinical development. Here we report structure-guided optimization of an amphipathic peptide, arenicin-3, originally isolated from the marine lugworm Arenicola marina. The peptide induces bacterial membrane permeability and […]
    Alysha G Elliott
  • A decade of advances in transposon-insertion sequencing June 14, 2020
    It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which combine genome-wide transposon mutagenesis with high-throughput sequencing to estimate the fitness contribution or essentiality of each genetic component in a bacterial genome. Four TIS variations were published in 2009: transposon sequencing (Tn-Seq), transposon-directed insertion site sequencing (TraDIS), insertion sequencing (INSeq) […]
    Amy K Cain
  • Identification of genes required for the fitness of <em>Streptococcus equi</em> subsp. <em>equi</em> in whole equine blood and hydrogen peroxide April 2, 2020
    The availability of next-generation sequencing techniques provides an unprecedented opportunity for the assignment of gene function. Streptococcus equi subspecies equi is the causative agent of strangles in horses, one of the most prevalent and important diseases of equids worldwide. However, the live attenuated vaccines that are utilized to control this disease cause adverse reactions in […]
    Amelia R L Charbonneau
  • The Role of Zinc Efflux during <em>Acinetobacter baumannii</em> Infection October 29, 2019
    Acinetobacter baumannii is a ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised individuals. The success of A. baumannii is partly attributable to its high level of antibiotic resistance. Further, A. baumannii expresses a broad arsenal of putative zinc efflux systems that are likely to aid environmental persistence and host colonization, but detailed […]
    Saleh F Alquethamy
  • Complete Genome Sequence of Pseudomonas aeruginosa Reference Strain PAK October 12, 2019
    We report the complete genome of Pseudomonas aeruginosa strain PAK, a strain which has been instrumental in the study of a range of P. aeruginosa virulence and pathogenesis factors and has been used for over 50 years as a laboratory reference strain.
    Amy K Cain